Denison is accredited by the Higher Learning Commission (hlcommission.org), an institutional accreditation agency recognized by the U.S. Department of Education. Denison is certified by the Ohio Board of Regents to grant three degrees: Bachelor of Arts, Bachelor of Science, and Bachelor of Fine Arts.

Denison's program in chemistry is accredited by the American Chemical Society.

Denison is a member of the National Association of Independent Colleges and Universities, the Great Lakes Colleges Association, the Association of Independent Colleges and Universities of Ohio, and several additional national and state associations. The college participates in the North Coast Athletic Conference of NCAA Division III.